Insulin increased by 39% the recovery of carbon-C14 from uniformly labeled glucose in glycogen in rabbit liver slices incubated in a high-potassium buffer, but did not affect the recovery of glucose carbon in CO2 or total lipid. Since the pathways to CO2 and lipid were not relatively more saturated than that to glycogen in livers incubated under conditions identical to those in which the insulin effect was observed, the insulin effect is assumed to be specific for glycogen synthesis. Insulin had no effect on glucokinase activity in incubated rabbit liver slices during the time observed (1 hr), nor on the recovery of labeled glucose as any of the measured metabolic products after incubation in Krebs-Ringer buffer.
- glucose metabolism
- ionic effect on insulin action
- Copyright © 1965 by American Physiological Society