Ca++ and pancreatic amylase release

The effects of Ca'++ on release of amylase by mouse pancreas in vitri was studied.The cholinergic agonist bethanechol was depressed about 50% in O Ca'++ medium.When pancreatic fragments were superfused with O Ca'++ medium, bethanechol still produced a normal stimulation of amylase release after half of the total tissue Ca'++ was washed out. This Ca'++ loss included both extracellular and some intracellularCa'++. Uptake and equilibration of '45Ca'++ into pancreatic fragments was multiphasic, with total equilibration with stable Ca'++ still not reached after 3 h. Addition of bethanechol had no effect on the rate of '45Ca uptake and equilibration. '45Ca'++ efflux was not influenced by superfision in O Ca'++ medium, while thestimulation of '45Ca'++ efflux by bethanechol was enhanced. It is concluded that extracellular Ca'++ and/or Ca'++ influx is not of major importance in triggering pancreatic enzyme release, but that extracellular Ca'++ may regulate the release process thus accounting for the parallel changes in unstimulated and stimulated amylase release.

This article has been cited by other articles:

				K. C. Swanson, J. C. Matthews, C. A. Woods, and D. L. Harmon Influence of substrate and/or neurohormonal mimic on in vitro pancreatic enzyme release from calves postruminally infused with partially hydrolyzed starch and/or casein J Anim Sci, May 1, 2003; 81(5): 1323 - 1331. [Abstract] [Full Text] [PDF]