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Am J Physiol 208: 9-13, 1965;
0002-9513/65 $5.00
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Intrinsic factor-like activity in extracts of guinea pig intestine

Shigeo Ukyo 1 and Bernard A. Cooper 1

1 Hematology Service, Department of Medicine, Royal Victoria Hospital, and McGill University Clinic, Montreal, Canada

Co57-labeled B12 tube fed to guinea pigs with normal human gastric juice has been found in both the supernatant and sediment of a saline extract of intestinal mucosa. The Co57-labeled material in the supernatant was excluded from Sephadex G-25, was not ultrafiltrable, and functioned as an intrinsic factor-B12-Co57 complex in an in vitro assay for intrinsic factor activity.

Key Words: vitamin B12 transport

Submitted on July 20, 1964






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