Action of natural angiotensin II and synthetic analogues on strips of rabbit aorta

¹ Lilly Laboratory for Clinical Research, Marion County General Hospital, and Departments of Experimental Medicine and Biochemistry, Indiana University School of Medicine, Indianapolis, Indiana

The pressor activity of pure natural asp¹ileu⁵-angiotensin II, and synthetic asparginyl¹-val⁵, asp¹-val⁵, asp¹-ileu⁵, and ß-asp¹-val⁵-angiotensin II was determined in a nephrectomized pithed cat. All were of equal potency with the exception of ß-asp¹-val⁵-angiotensin which was slightly more active. When assayed on the aortic strip asparginyl¹-val⁵ had 25% and ß-asp¹-val⁵-angiotensin had 75% of the activity of natural angiotensin. The difference in action of the angiotensin peptides can not be explained by their rate of destruction by peptidases. Marked variation in the specificity of different receptors might be responsible. Asparginyl¹-val⁵-angiotensin II should not be used as a standard in assaying natural angiotensin unless the specificity of the test system has been determined.

Note:
With the Technical Assistance of R. M. Sanders

Key Words: asp¹-ileu⁵-angiotensin II • asp¹-val⁵-angiotensin II • asparginyl¹-val⁵-angiotensin II • ßbeta;-asp¹-val⁵-angiotensin II

Submitted on July 1, 1963

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