Na-K activated adenosine triphosphatase formation of cerebrospinal fluid in the cat

¹ National Institute of Neurological Diseases and Blindness, and National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Digitalis-sensitive Na-K activated ATPase (Na-K ATPase), implicated in active cation transport, was shown to occur in cat choroid plexus. Cerebrospinal fluid (CSF) formation rate, measured by collection from cat cerebral aqueduct was inhibited 18% by intravenous desacetyl lanatoside C (0.2 mg/kg). Ouabain, placed intraventricularly, caused inhibition of flow, ranging from 100% by 10^–5 mole to 0% by 5 x 10^–10 mole. After 10^–6 mole ouabain, Na-K ATPase activity in lateral ventricle choroid plexus was inhibited 69% without change in digitalis-insensitive, Mg activated ATPase and carbonic anhydrase activities. Ventriculocisternal perfusion with varying concentrations of ouabain, scillaren A, and hexahydroscillaren A gave CSF flow inhibition (inulin dilution technique) correlating quantitatively with in vitro Na-K ATPase inhibition. Other compounds studied were erythrophleine, cassaine, and l-norepinephrine. It is concluded that the choroid plexus Na-K ATPase system has a primary function in the formation of CSF in the cat, presumably through the active secretion of Na ions into the ventricle.

Note:
With the Technical Assistance of Mel Rose Canady, Naomi M. Hawkins, Leo L Caravaggio, and Howard Faden

Key Words: carbonic anhydrase • digitalis • ouabain • desacetyl • lanatoside C • scillaren A • hexahydroscillaren A • erythrophleine • cassaine • choroid plexus

Submitted on October 4, 1963

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