Effects of inhibitors on contractions of normal and dystrophic mouse muscles

¹ Division of Physiology, Institute for Muscle Disease, Inc., New York City

Exposure to 0.05 mm 2,4-dinitrophenol (DNP), 1 mm NaN₃, and 1 mm KCN reveals the following differences between normal and dystrophic excised, curarized gastrocnemius muscles of mice of strain 129. DNP briefly enhances twitch but not tetanus tension in rested dystrophic mice before depressing both tensions. In rested normal mice these tensions decline immediately. NaN₃ reduces tensions in rested normal mice for 30 min; slow recovery follows. Half-relaxation rate is also reduced. In rested dystrophic mice tension does not recover from depression in NaN₃, and the relaxation rate increases. During fatigue, tension output in dystrophic declines less rapidly than in normal mice, regardless of inhibitors. NaN₃ accentuates fatigue-associated increases of contraction rate in dystrophic mice, but accelerates the usual reduction of this rate in normal ones. The two muscle groups also show quantitative differences in their responses to the inhibitors. Except for some DNP effects, all changes are reversible. Known actions of the inhibitors account only inadequately for these observations, and no clearly identifiable metabolic lesions of dystrophic muscles emerge.

Key Words: muscular dystrophy • mouse skeletal muscle • effects of NaN₃ • effects of KCN • effects of 2, 4-dinitrophenol • changes in contraction variables due to metabolic inhibitors

Submitted on June 17, 1963