Measurement of short-circuit current and ion transport across the ileum

¹ Department of Radiation Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York

An apparatus is described which allowed control over both the electrical and chemical potential gradients across the isolated ileum of the rat and at the same time permitted the measurement of the transport of ions and water. Silver ions, released from the electrodes, affected ion transport. In the absence of complexing agents, the short-circuit current (SCC) fell during the 60-min measurement period and was equal to the net sodium transport. With 10^–3 m reduced glutathione in the bathing solution, the SCC was constant and equal to 94% of the sodium transport. With 10^–4 m cysteine, the SCC was greatly increased. Sodium transport, also increased, was equal to 75% and chloride secretion to 20% of the SCC. A mechanism by which silver ions stimulate sodium transport, is proposed. Comparison of water and salt transport between open- and short-circuited tissues indicated that chloride ions were more effective in producing water movement when transported from mucosal to serosal solutions directly through the tissue (open circuit) rather than via the Ag/AgCl electrode system (short-circuit experiments).

Key Words: short-circuit apparatus • sodium transport • chloride transport • water transport • active transport • chloride secretion • effect of silver on sodium transport

Submitted on May 23, 1963

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