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Am J Physiol 206: 218-222, 1964;
0002-9513/64 $5.00
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Relation between hydrogen ion secretion and oxygen uptake by gastric mucosa

J. G. Forte 1 and R. E. Davies 1

1 Department of Physiology, School of Medicine, and Department of Animal Biology and Molecular Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

Bullfrog gastric mucosae were isolated, mounted between two glass chambers, and bathed with physiological salt solutions equilibrated with 5% CO2 and 95% O2. Oxygen consumption (qO2; measured polarographically) and acid secretion (qH+; pH stat method) were measured along with the transmucosal potential difference (p.d.) and current passing through the mucosa. Histamine (4 x 10–4 m) caused an increase in qH+ and qO2. In measurements on nine short-circuited mucosae the mean ratio for the DeltaqH+/DeltaqO2 was 2.1. Sodium thiocyanate (0.5–15 mm) caused a decrease in qH+ and qO2 and an increase in short-circuit current. These effects were reversible. The ratio of DeltaqH+/DeltaqO2 induced by thiocyanate varied from 5.0 to 12.0. Current (0.5 to 1.0 ma/cm2) passed through the mucosae, which reversed the normally observed p.d. to values between +70 and +240 mv (secretory side with respect to nutrient side in an external circuit), caused a decrease in qH+ and qO2; the average DeltaqH+/DeltaqO2 was approximately 13. Using either thiocyanate or electric current the ratio of the induced DeltaqH+/DeltaqO2 can really exceed 4.0, the electrochemical equivalent of oxygen, and thus if this extra oxygen provides the energy for the extra acid secretion these results invalidate a simple redox pump hypothesis of hydrogen ion transport by gastric mucosa.

Note:
With the Technical Assistance of Patricia Adams

Key Words: gastric acid secretion • hydrochloric acid secretion by the stomach • thiocyanate inhibition of acid secretion • redox pump hypothesis of active transport • changes in acid secretion induced by electric current • short-circuit current and thiocyanate in gastric mucosa • active transport of ions by gastric mucosa • thermodynamic and stoichiometric relations in active transport

Submitted on July 23, 1963






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