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Am J Physiol 205: 1099-1105, 1963;
0002-9513/63 $5.00
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Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from human gastric mucosa

Max J. Seijffers 1, Harry L. Segal 1, and Leon L. Miller 1

1 Departments of Medicine and Biochemistry, the University of Rochester School of Medicine and Dentistry, Rochester, New York

Extracts of human gastric mucosa have been fractionated on diethylaminoethyl cellulose to yield three precursors of proteolytic enzymes active at low pH which have been called pepsinogen I, pepsinogen II, and pepsinogen III. Pepsinogen I has been found in the mucosa from all parts of the stomach examined as well as in the proximal duodenum. Pepsinogen II and pepsinogen III were present in fundic mucosa only.

Key Words: fractionation • DEAE-cellulose

Submitted on June 13, 1963






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