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Am J Physiol 202: 981-983, 1962;
0002-9513/62 $5.00
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Phosphate release from dog erythrocytes

John M. Ginski 1, John F. Thomson 1, and Akira Omachi 1

1 Division of Biological and Medical Research, Argonne National Laboratory, Argonne; and Department of Physiology, Stritch School of Medicine, Loyola University, Chicago, Illinois

P32 release from dog erythrocytes previously incubated with radioactive inorganic phosphate was investigated. Under both aerobic and anaerobic conditions, apparent activation energies of similar magnitude, 25,000 cals/mole, were obtained. However, P32 release was lower in the absence of oxygen and greater in the presence of iodoacetate. These results suggest that the cellular pool from which P32 is released is accessible to glycolytic enzymes and that the rate of P32 release is related in some manner to the orthophosphate concentration in the precursor pool.

Submitted on November 8, 1961






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