In vitro I¹³¹ uptake by salivary glands, stomach and placenta

¹ Department of Physiology and Pharmacology, Michigan State University, East Lansing, Michigan

Slices of various tissues of the rat were incubated at 37°C for 6 hours in 2 ml of medium containing 0.3 µc carrier-free I¹³¹. I¹³¹ concentration significantly above that of the medium, and of boiled tissues, was observed in salivary glands and stomach, but not in liver or placenta. I¹³¹ uptake by parotid was significantly higher than that of submaxillary and sublingual tissues. Uptake by tissues from the cardiac portion of the stomach was significantly greater than in those from the pyloric portion. Concentration of I¹³¹ by salivary glands and stomach slices was reduced by the addition of thiouracil (0.02 mg/ml), KSCN (0.1 mg/ml) or NaCN (0.1 mg/ml) but it was not affected by the addition of TSH (100 µg/ml) to the medium. None of these additives influenced the small I¹³¹ uptake found in liver and placenta. I¹³¹ uptake by salivary glands and stomach of the mouse, dog and cow was similar to that in the rat, except that there was less difference between the parotid and submaxillary glands.

Note:
With the Technical Assistance of H. W. Kaczkofsky