Accumulation of Ca⁴⁵ by slices of the small intestine

¹ Department of Medicine, Columbia University College of Physicians and Surgeons; and the Presbyterian Hospital, New York City

Respiring slices of the proximal small intestine of the rat accumulate Ca⁴⁵ in vitro, and after 1 hour of incubation at 37°C the Ca⁴⁵ concentration ratio tissue/medium is approximately 5. When oxidative metabolism is prevented by incubation under N₂, or when the generation of phosphate-bond energy is inhibited with 2,4-dinitrophenol, the corresponding Ca⁴⁵ ratio tissue/medium is approximately 2. The oxygen-dependent accumulation of Ca⁴⁵ is limited in capacity, relatively specific for Ca⁺⁺ in contrast to Sr⁺⁺ or Ba⁺⁺, inhibited by K⁺, and dependent on the dietary vitamin D. It is greater with slices from young, growing rats than with those from older rats. The results indicate that the accumulation of Ca⁴⁵ by slices results mainly from the active transfer of calcium previously observed with everted gut-sacs.

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