Alkaline Phosphatase Activity in Various Mouse Tissues Following Total Body X-Irradiation

¹ From the Cancer Research Laboratory, Department of Hospitals, City of New York and the Department of Biology, Graduate School of Arts and Sciences, New York University, New York City

The distribution of alkaline phosphatase in various mouse tissues (duodenum, spleen, liver, kidney and mammary glands) and the effects of ionizing radiation on the activity and distribution of this enzyme were studied by the use of the Gomori method. A total-body dose of 700 r, known to kill 50% of the mice of the C57/BL6 strain in about 9 days, as well as doses up to 10,000 r, failed to produce any apparent change in alkaline phosphatase activity of the various tissues which were removed from 2 hours to 14 days after exposure. On the basis of these observations it may be inferred that alkaline phosphatase is not involved in cytological damage induced by ionizing radiation.

Note:
with the assistance of Grace E. Clarke