In Vitro Effects of Acetate Ion on Renal Metabolism of p-Aminohippurate

¹ From the Section on Clinical Endocrinology, Clinic of General Medicine and Experimental Therapeutics, National Heart Institute, National Institutes of Health, Bethesda, Maryland

Renal cortical slices from four strains of rats were incubated with p-aminohippurate (PAH) and the concentration gradient for PAH (S/M PAH) was determined. Acetate ion (Ac) at a concentration of 0.01 m failed to induce the expected increase in S/M PAH. At larger concentrations of Ac, the S/M PAH was depressed. Maximal depression occurred when the concentration of Ac was 0.1 m. Ac produced a concurrent increase in the respiratory rate, an increase in the terminal tissue weight, and no significant alteration of the rate of N-acetylation of PAH.

Note:
with the technical assistance of Catherine Delea